The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM)
The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth.
Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more.
- Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value
- Provides insight into the design and development philosophy behind current instrument manufacturers
- Covers sample handling, applications, and key supporting techniques
- Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research
- Presented in full colour
An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.
1. Scanning Electron Microscopy: Theory, history and development of the field emission scanning electron microscope
2. Akashi Seisakusho Ltd – SEM Development 1972 - 1986
3. Development of FE-SEM technologies for life science fields
4. A History of JEOL Field Emission Scanning Electron Microscopes with reference to biological applications
5. TESCAN Approaches to Biological Field Emission Scanning Electron Microscopy
6. FEGSEM for Large Volume 3D Structural Analysis in Life Sciences
7. ZEISS scanning electron microscopes for biological applications
8. SEM Cryo-stages and preparation chambers
9. Cryo–SEM Specimen Preparation Workflows from the Leica Microsystems Design Perspective
10. Chemical Fixation
11. A Brief Review of Cryobiology with reference to cryo Field Emission Scanning Electron Microscopy
12. High-Resolution Cryo Scanning Electron Microscopy of Macromolecular Complexes
13. FESEM in the examination of mammalian cells and tissues.
14. Public Health/ Pharmaceutical Research – Pathology and Infectious disease
15. Field emission scanning electron microscopy in cell biology featuring the plant cell wall and nuclear envelope
16. Low voltage scanning electron microscopy in yeast cells
17. Field emission scanning electron microscopy in food research.
18. Cryo-FEGSEM in Biology
19. Preparation of Vitrified Cells for TEM by Cryo-FIB Microscopy
20. Environmental Scanning Electron Microscopy
21. Correlative Array Tomography
22. The Automatic Tape Collection UltraMicrotome (ATUM)
23. SBEM techniques
24. FIB-SEM for Biomaterials
25. New opportunities for FIB/SEM EDX in nanomedicine: cancerogenesis research
26. FIB-SEM Tomography of Biological Samples: Explore the Life in 3D
27. Three-dimensional Field-Emission Scanning Electron Microscopy as a Tool for Structural Biology
28. Element analysis in the FEGSEM application and limitations for biological systems.
29. Image and Resource Management in Microscopy in the digital age
30. Part 1: Optimizing the Image Output: Tuning the SEM parameters for best photographic results
Part 2: Post processing of the photomicrograph
31. A Synoptic View on Microstructure: Multi-Detector Colour Imaging, nanoflight®